A SELENIUM-DEPENDENT GLUTATHIONE PEROXIDASE IN THE JAPANESE SCALLOP MIZUHOPECTEN YESSOENSIS CDNA CLO

来源 :18th International Pectinid Workshop(第18届国际扇贝研讨会) | 被引量 : 0次 | 上传用户:loupee
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  Glutathione peroxidase (GPx) is an antioxidant enzyme that protects cells from oxidative damage in the innate immune responses against bacterial infections.In this study, we report cloning and characterization of a GPx (designated as MyGPx) coding sequence and promoter in Japanese scallop, Mizuhopecten yessoensis.The full-length 1081 nt MyGPx mRNA contained a 28 nt 5untranslated region (UTR), a 603 nt open reading frame and a 450 nt 3UTR containing a polyadenylation signal (AATAAA).Multiple sequence alignment revealed that amino acids essential to enzymatic function of MyGPx proteins were highly conserved.A 1628 nt 5-flanking sequence of MyGPx was identified by genome walking.
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