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Predictably assembling genetic circuits requires the compositional DNA parts encoding defined and fixed functions.However,the defined functions of a part are usually interfered by other parts and their host cells.Several methods have been developed to insulate the parts from their genetic context.Here,we describe a modular architecture to insulate the operators from their cognate promoters (recognized by T7 RNAP or sigma ECF11) in E.coli.In the modular architecture,the regulatory functions of operators were not substantially affected by the cognate promoters;meanwhile promoters activities were also independent of the operators.More than 1000 transcriptional regulatory parts could be easily constructed and predicted by a simple model without any fitting parameter.