【摘 要】
：
This study presents the effects of different carbon sources on the electricity generation ability of microorganisms investigated by the membraneless μMFC developed in our previous report [1] (Fig.1).A
【机 构】
：
Department of Chemical Engineering,National Cheng Kung University,Tainan,Taiwan
【出 处】
：
第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议
论文部分内容阅读
This study presents the effects of different carbon sources on the electricity generation ability of microorganisms investigated by the membraneless μMFC developed in our previous report [1] (Fig.1).Although lab-scale MFCs are able to identify the microbial for electricity generation,they require several days for the detection [2].The membranelessμ MFC can accomplish the analysis in as short as 30 min.In this study,several carbon sources such as sodium acetate,glucose and sucrose were applied to investigate their effects on the electricity generation ability of mixed-culture microorganisms.The membranelessμMFC includes the a Y-shaped channel and two flat gold microelectrodes on the glass slide.The flow channel was made by PDMS via soft lithography and the flat gold electrode was fabricated by E-beam evaporation and wet etching.The PDMS sheet and the slide were bonded by O2 plasma treatments.Alive/active cells generate electrons during metabolic process but inactivated cells are not able to carry out the above reactions.Therefore,the difference between active and inactivated samples can be applied to verify the amount of electrons generated by metabolism.Sucrose as a carbon source generated the highest open circuit voltage,which was about 190 mV (Fig.2).Acetate and glucose as carbon sources generated about 50 and 60 mV,respectively.Therefore,sucrose is a more suitable carbon source for the current mixed-culture microorganisms to produce electricity.Using similar principles,this membranelessμMFC can be applied to screening suitable carbon sources for specific microorganism strain or screening cultivation parameters for improving the performance of lab-scale or commercial-scale MFCs.
其他文献
Generally speaking,drugs can be delivered into human body through different routes of administration,among which injection and oral delivery are the most commonly used.However,injection cannot be easi
Stem cell microenvironments are enriched by signals from a variety of components,which cooperate spatially and temporally to regulate cellular function [1].In vitro recapitulating such complexity in a
Chondrocytes are highly specialized cells of mesenchymal origin that are responsible for producing,sustaining and degrading the cartilage extracelluar matrix (ECM)[1].The two main macromolecules in ar
Miniaturized biosensors and bioanalytical systems promise to revolutionize the field of health care and personalized medicine,in light of sample reduction,speed,and sensitivity.However,mass transport
Optofluidics aims in manipulating light and fluid at microscale and exploiting their interaction to create highly versatile devices that have significant scientific interests in many areas.The novelti
With the development of microfluidics,a new research area called nanofluidics appears.As the channel size deceases from micrometer scale to nanometer size,especially to the size of electric double lay
This paper describes two microfluidic devices for cell and protein analysis.One device is made from glass,and it contains unique geometry and design of micropillars.1 As shown in Figure 1,this device
In this study,patterning of fibroblasts,NIH-3T3 cells,by alternating current dielectrophoresis (ACDEP) is performed to make the electrotaxis observation much easier and more effective.The movement of
There is an emerging need in the fields of proteomics,cellomics and early diagnosis to selectively analyze targeted proteins at the single-molecule level [1,2].A nanochannel,as an appropriate space,is
Microfluidic biosynthesis of Au–Ag bimetallic nanoparticle in a tubular microreactor based on simultaneous reduction of HAuCl4 and AgNO3 precursors in the presence of Cacumen Platycladi (C.Platycladi)