【摘 要】
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This paper describes two microfluidic devices for cell and protein analysis.One device is made from glass,and it contains unique geometry and design of micropillars.1 As shown in Figure 1,this device
【机 构】
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Department of Mechanical and Aerospace Engineering;Pruitt Family Department of Biomedical Engineerin
【出 处】
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第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议
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This paper describes two microfluidic devices for cell and protein analysis.One device is made from glass,and it contains unique geometry and design of micropillars.1 As shown in Figure 1,this device is coated with either DNA aptamers or antibodies and then used for the isolation of circulating tumor cells (CTC) in peripheral blood.The device consists of >59,000 micropillars,which enhance the probability of the interactions between cancer cells and the capture reagents on pillar surfaces.We applied the platform to blood analysis,and demonstrated the detection of 10 cancer cells spiked in 1 mL of blood.The device was capable of processing 1 mL of blood within 28 minutes.Further,we have applied the device to clinical samples,and the device is able to identify CTC according to FDA-approved definition.The second device is made from cyclic olefin copolymer (COC).The device is designed for two-dimensional gel electrophoresis (2DGE).2DGE consists of isoelectric focusing (IEF) as the first dimension and polyacrylamide gel electrophoresis (PAGE) as the second dimension.One major advantage of 2DGE is its enormous separation resolution,whereas its key limitations include poor reproducibility and time-consuming procedure.The device is designed to address the limitations.As shown in Figure 2,the device consists of multiple channels,rather than a single channel,for the second dimension to match the separation capacity of the slab in 2DGE.2 The key advancement of the device are twofold: (1) an array of microfluidic valves allows the seamless introduction of two different separation media; and (2) a fluidic network with densely-packed channels enables the transfer of proteins from the first to the second dimension.We demonstrated the separation of protein biomarkers associated with traumatic brain injury.
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