【摘 要】
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There is an emerging need in the fields of proteomics,cellomics and early diagnosis to selectively analyze targeted proteins at the single-molecule level [1,2].A nanochannel,as an appropriate space,is
【机 构】
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Nanoscience and Nanotechnology Research Center,Research Organization for the 21st Century,Osaka Pref
【出 处】
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第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议
论文部分内容阅读
There is an emerging need in the fields of proteomics,cellomics and early diagnosis to selectively analyze targeted proteins at the single-molecule level [1,2].A nanochannel,as an appropriate space,is expected to develop novel tools for molecule analysis at the single-molecule level [3].We have proposed a novel concept of nanochannel-based single-molecule immunoassays [4],as shown in Figure 1.In a confined nanochannel space however,patterning molecules (capture antibodies),a common core step of immunoassay methods,is extremely difficult.Recently,we developed a fabrication position control technique with a super high precision which is smaller than 50 nm.With this novel technique,high-precision gold nanopatterns with various characteristic sizes were directly fabricated inside nanochannels for the first time (Figure 2).Through formation of self-assembled monolayers (SAMs) having carboxyl headgroups on the surface of the gold nanopatterns,high-precision nanopatterning of proteins in the nanochannel was achieved (Figure 2).
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