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目的:建立高效液相色谱波长切换法对枳芍散水煎液中4个成分(芍药苷、柚皮苷、橙皮苷、新橙皮苷)进行分析。方法:采用Syncronis C18(4.6 mm×250 mm,5μm)色谱柱,以0.3%磷酸水溶液(A)-乙腈(B)为流动相,二元梯度洗脱,流速1.0 mL.min-1,检测波长为230 nm(0～30 min测定芍药苷)、283 nm(30～60 min测定柚皮苷、橙皮苷、新橙皮苷)。结果:枳芍散中4个成分芍药苷、柚皮苷、橙皮苷、新橙皮苷进样量分别在0.12～1.73μg(r=0.999 6),0.38～5.68μg(r=0.999 9),0.64×10-1～0.96μg(r=0.999 9),0.72～10.83μg(r=0.999 7)呈良好线性关系;平均回收率分别为104.17%,100.98%,103.72%,101.01%。结论:该方法准确可靠、重复性好,可用于枳芍散的质量控制。
OBJECTIVE: To establish a method for the determination of four components (paeoniflorin, naringin, hesperidin, neohesperidin) in the decoction of Hovenisch as Radix by HPLC with wavelength-switching method. METHODS: The mobile phase was eluted with a gradient of Syncronis C18 (4.6 mm × 250 mm, 5 μm) using 0.3% aqueous phosphoric acid (A) -acetonitrile (B) as a mobile phase with a flow rate of 1.0 mL · min- Paeoniflorin was measured at 230 nm (0-30 min) and naringin, hesperidin, neohesperidin at 283 nm (30-60 min). Results: The contents of paeoniflorin, naringin, hesperidin and neohesperidin in Zhushao powder were 0.12-1.73μg (r = 0.999 6), 0.38-5.68μg (r = 0.999 9) , 0.64 × 10-1 ~ 0.96μg (r = 0.999 9), 0.72 ~ 10.83μg (r = 0.999 7). The average recoveries were 104.17%, 100.98%, 103.72% and 101.01% respectively. Conclusion: The method is accurate, reliable and reproducible. It can be used for the quality control of Zhishishao San.