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目的 采用国际上常用的两种测定神经生长因子活性方法对中华眼镜蛇毒神经生长因子活性作用进行观察。方法 鸡胚背根神经节培养法 ,PC12细胞培养法。结果 鸡胚背根神经节培养法在样品浓度达 5 0ng/ml时有活性反应 ,PC12细胞培养法在定性测定 ,样品浓度为 2 0ng/ml时能观察到活性 ,在用定量测定方法时 ,实验组 ( 5ng/mlNGF)细胞聚团并长出神经状纤维 ,说明有活性。结论 鸡胚背根神经节培养法灵敏度低 ,操作复杂 ,但条件要求简单。PC12细胞培养法灵敏度较高 ,但条件要求也高 ,并且需要有可靠的细胞株。
Objective To observe the effects of nerve growth factor (NGF) activity using two commonly used methods for measuring nerve growth factor activity in the world. Methods Chick embryo dorsal root ganglion culture method and PC12 cell culture method. Results The culture of chicken embryo dorsal root ganglion was activated when the sample concentration reached 50ng / ml. The activity of PC12 cell culture was observed when the concentration was 20ng / ml. In the method of quantitative determination, The experimental group (5ng / mlNGF) cells agglomerate and grow neurofibers, indicating that the activity. Conclusion The chicken embryo dorsal root ganglion method has low sensitivity and complicated operation, but the requirements are simple. PC12 cell culture method is more sensitive, but the conditions are high, and the need for a reliable cell line.