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[目的]研究三苯氧胺(TAM)联合低剂量苦参碱对人乳腺癌Bcap-37细胞的增殖抑制及作用机制。[方法]用MTT法检测各组细胞的抑制率,克隆形成实验检测对细胞增殖的影响,流式细胞仪检测细胞凋亡率,Western blot法检测p53、Bax、Bcl-2蛋白表达。[结果]TAM对Bcap-37细胞增殖具有明显抑制作用,且具有剂量和时间依赖性,与低剂量苦参碱联合,其抑制作用明显增强。药物作用24、48、72h后,与对照组相比,TAM组的凋亡率均明显增高(P<0.01);与TAM组相比,联合组的凋亡率均明显增高(P<0.01)。Western blot检测发现,与对照组相比,TAM组p53、Bax蛋白表达增加,Bcl-2蛋白表达降低;与TAM组相比,联合组p53、Bax蛋白表达增加,Bcl-2蛋白表达降低。[结论]低剂量苦参碱能促进TAM对Bcap-37细胞增殖抑制作用,其作用机制与增加p53、Bax蛋白表达,降低Bcl-2蛋白表达,促进细胞凋亡有关。
[Objective] To investigate the inhibitory effect of tamoxifen (TAM) combined with low dose matrine on the proliferation of human breast cancer Bcap-37 cells and its mechanism. [Methods] The inhibitory rate of cells in each group was detected by MTT assay. The effect of clone formation test on cell proliferation was detected. The apoptosis rate was detected by flow cytometry. The protein expressions of p53, Bax and Bcl-2 were detected by Western blot. [Results] TAM had a significant inhibitory effect on the proliferation of Bcap-37 cells in a dose-and time-dependent manner. Combined with low dose of matrine, TAM significantly enhanced its inhibitory effect. Compared with the control group, the apoptotic rates of TAM group were significantly increased (P <0.01) after 24, 48, and 72 hours of drug treatment. Compared with TAM group, the apoptosis rate of TAM group was significantly increased (P <0.01) . Western blot showed that the expression of p53 and Bax increased and the expression of Bcl-2 decreased in TAM group compared with control group. Compared with TAM group, p53 and Bax protein increased and Bcl-2 protein decreased in TAM group. [Conclusion] Low dose matrine can promote TAM to inhibit the proliferation of Bcap-37 cells. Its mechanism may be related to increasing the expression of p53 and Bax, decreasing the expression of Bcl-2 and promoting apoptosis.