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目的分析2009-2013年深圳市流感病毒分离情况及流行特点,了解流感病毒优势株的情况,为流感防控提供科学依据。方法收集深圳市监测哨点的流感样病例咽拭子标本,用狗肾传代细胞(MDCK)培养分离流感病毒,经血凝抑制试验鉴定病毒型别,并对病原学结果和暴发疫情进行描述性分析。结果 2009-2013年共检测咽拭子标本20 579份,分离出流感病毒2 939株,总检出率为14.28%,其中甲型H1N1亚型969株(32.97%),季节性H1N1亚型140株(4.76%),H3N2亚型939株(31.95%),B-Victoria亚型710株(24.16%),Yamagata亚型181株(6.16%)。5年的优势流行株依次为H3N2亚型、B-Victoria亚型、新甲H1N1型、H3N2亚型和新甲H1N1型。2009年总检出率最高占44.44%,2011年最低占8.98%。2009-2013年共报告896起流感样病例暴发疫情,流感PCR检测阳性820起,以新甲型H1N1型和乙型为主,分别占39.76%和39.51%,季节性甲型和乙型混合感染10起占1.22%。结论深圳市流感流行优势毒株在H3N2亚型、B-Victoria亚型、新甲H1N1型之间交替出现,同时存在多种流感病毒交叉流行现象,3月和6月出现流行高峰。
Objective To analyze the isolation and prevalence of influenza virus in Shenzhen from 2009 to 2013 and to understand the prevalence of influenza virus dominant strains so as to provide a scientific basis for prevention and control of influenza. Methods The throat swab samples of influenza-like cases collected from the sentinel surveillance in Shenzhen were collected, and the influenza virus was isolated from the MDCK cells. The virus type was identified by the hemagglutination inhibition test. The etiological and outbreaks were characterized descriptively analysis. Results A total of 20 579 samples of throat swabs were detected in 2009-2013. A total of 2 939 influenza virus isolates were detected with a total detection rate of 14.28%, of which 969 (32.97%) were type A H1N1 subtypes, and the seasonal H1N1 subtype 140 (4.76%), 939 (31.95%) H3N2 subtypes, 710 (24.16%) B-Victoria subtypes and 181 (6.16%) Yamagata subtypes. The five-year prevalent strains were H3N2 subtype, B-Victoria subtype, new H1N1 subtype, H3N2 subtype and new H1N1 subtype. In 2009 the highest total detection rate of 44.44%, the lowest in 2011 accounted for 8.98%. A total of 896 flu-like outbreaks were reported in 2009-2013. The number of positive influenza PCR tests was 820, accounting for 39.76% and 39.51% of the total respectively, with seasonal influenza A and B infections 10 accounted for 1.22%. Conclusion Influenza prevalence strains in Shenzhen alternate between H3N2 subtype, B-Victoria subtype and New H1N1 subtype. At the same time, there are a number of influenza virus cross-epidemic phenomena, with a peak in March and June.