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目的探讨C-X-C趋化因子配体12α(CXCL12α)和CXCL12β对肝星状细胞迁移的影响。方法体外培养LX-2肝星状细胞,分为正常对照组、10ng/mL血小板衍生生长因子BB(PDGF-BB)组、(50、100、200)ng/mL CXCL12α组、(50、100、200)ng/mL CXCL12β组。采用TranswellTM法检测CXCL12α和CXCL12β对LX-2细胞迁移能力的影响;外源性CXCL12刺激LX-2细胞,用Western blot法检测C-X-C趋化因子受体4(CXCR4)蛋白的表达。结果 TranswellTM实验结果显示各组迁移的LX-2细胞个数分别为:正常对照组(66.33±11.43)、PDGF-BB组(127.47±31.68)、50ng/mL CXCL12α组(106.13±12.94)、100ng/mL CXCL12α组(125.87±17.00)、200ng/mL CXCL12α组(137.07±21.03)、50ng/mL CXCL12β组(103.80±11.26)、100ng/mL CXCL12β组(122.33±19.46)、200ng/mL CXCL12β组(124.40±15.16)。CXCL12α和CXCL12β均可促进LX-2细胞迁移;且随着CXCL12α和CXCL12β浓度增加,LX-2细胞迁移增强。LX-2细胞可表达CXCR4蛋白,外源性CXCL12刺激LX-2细胞后,CXCR4蛋白表达无变化。结论 CXCL12α和CXCL12β均可促进肝星状细胞迁移。
Objective To investigate the effects of C-X-C chemokine ligand CXCL12α and CXCL12β on hepatic stellate cell migration. Methods LX-2 hepatic stellate cells were cultured in vitro and divided into normal control group, 10ng / mL platelet-derived growth factor BB (PDGF-BB) group, (50,100,200) ng / mL CXCL12α group, 200) ng / mL CXCL12β group. The effects of CXCL12α and CXCL12β on the migration of LX-2 cells were detected by TranswellTM assay. LX-2 cells were stimulated by exogenous CXCL12 and the expression of CXCR4 protein was detected by Western blot. Results The results of TranswellTM assay showed that the number of LX-2 cells in each group were 66.33 ± 11.43, 127.47 ± 31.68, 106.13 ± 12.94 and 100ng / mL (125.37 ± 17.00), 200ng / mL CXCL12α (137.07 ± 21.03), 50ng / mL CXCL12β (103.80 ± 11.26), 100ng / mL CXCL12β (122.33 ± 19.46) and 200ng / mL CXCL12β (124.40 ± 15.16). Both CXCL12α and CXCL12β promoted the migration of LX-2 cells, and the migration of LX-2 cells increased with the increase of CXCL12α and CXCL12β concentrations. CXCR4 protein was expressed in LX-2 cells, and CXCR4 protein expression was not changed in LX-2 cells stimulated by exogenous CXCL12. Conclusion Both CXCL12α and CXCL12β promote hepatic stellate cell migration.