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目的 研究丙型肝炎病毒(HCV)的致癌机理。方法 以免疫组化ABC法对46 例肝细胞癌(HCC)及其癌旁肝组织的HCV CP10、NS3及NS5 抗原和ras p21、c-m yc、c-erbB-2、突变型p53 及p16 蛋白进行染色,并将HCC分为HCV抗原阳性组和HCV 抗原阴性组,对比分析HCV 感染对上述癌相关基因蛋白产物表达的影响。结果 46 例HCC患者中,1～3 种HCV 抗原阳性20 例(43.5 % ),2～3 种HCV 抗原阳性16 例(34.8 % ),3 种HCV 抗原阳性9 例(19.6 % )。HCV 抗原阳性组p16 蛋白表达缺失率(80 % ,16/20)明显高于HCV 抗原阴性组(42.3 % ,11/26)(P<0.025),而其他癌相关基因产物在HCV 抗原阴阳性组间的表达则无明显差异。结论 重庆地区约三分之一左右肝癌的发生可能与HCV 感染有关;HCV 致癌的分子机制可能涉及细胞周期负性调节因子p16基因功能的受抑
Objective To study the carcinogenic mechanism of hepatitis C virus (HCV). Methods The HCV CP10, NS3 and NS5 antigens and the ras p21, cm yc, c-erbB-2, mutant p53 and p16 proteins in 46 cases of hepatocellular carcinoma (HCC) The HCCs were divided into HCV positive group and HCV negative group. The influence of HCV infection on the protein expression of cancer related genes was analyzed. Results Among the 46 patients with HCC, 1 to 3 HCV antigens were positive in 20 cases (43.5%), 2 to 3 HCV antigens were positive in 16 cases (34.8%) and 3 HCV antigens were positive in 9 cases (19.6%). The loss of p16 protein expression in HCV antigen positive group (80%, 16/20) was significantly higher than that in HCV negative group (42.3%, 11/26) (P <0.025), while the other cancer related gene products were negative in HCV antigen negative group Between the expression was no significant difference. Conclusions About one third of HCC occurrences in Chongqing may be related to HCV infection. The molecular mechanism of HCV carcinogenesis may involve suppression of p16 gene function of cell cycle negative regulator