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采用傅里叶变换红外光谱、二阶导数光谱、共有峰率和变异峰率的双指标序列等方法,首次对祖师麻中3种已知含量及功效的标准物质、不同提取方法提取的有效部位及渗漉液的不同萃取部位进行了结构特征的分析和鉴别。结果表明,祖师麻甲素及7-OH香豆素在1700cm~(-1)附近内酯及芳环在1600cm~(-1)附近有强吸收,紫丁香苷芳环v_C=c1589、1509cm~(-1)骨架振动及羟基v_(O-H)3563cm~(-1)的吸收峰较强。加热回流提取的有效部位在3300cm~(-1)处有较强的羟基V_(O-H)伸缩振动或氨基酸等v_(N-H)伸缩振动的叠加,渗漉法提取的有效部位在此处也有较强吸收峰,且3518cm~(-1)处的V_(N-H)较为明显。另外,两法提取的有效部位红外图谱共有峰率仅为45%,且变异峰率高达83.8%。其不同萃取部位的红外一维图谱均较好的体现了C=O、C—H、O—H、N—H及甲基、亚甲基的伸缩振动;对比各萃取部位的二阶导数谱,发现石油醚部除745—715cm~(-1)附近外,其余位置峰数明显少于其他3个萃取部位,乙酸乙酯部的峰数较多且峰形较好,氯仿和正丁醇部峰数与之相差不多,但峰形较弱,通过双指标法得出氯仿部和石油醚部所含物质种类相差极大,而氯仿部和乙酸乙酯部较为相近。
The three reference materials with known contents and efficacies in Zujianma were obtained by means of Fourier transform infrared spectroscopy, second derivative spectroscopy, double index sequence with common peak rate and mutation peak rate. The effective fractions extracted by different extraction methods And percolate liquid extraction of different parts of the structural characteristics of the analysis and identification. The results showed that lactone and 7-OH coumarin at 1700cm -1 had a strong absorption around 1,600cm -1 in the vicinity of 1,700cm -1. The syringicin aromatic ring v_C = c1589,1509cm ~ (-1) vibration and strong absorption peak at 3563cm ~ (-1) of hydroxyl v_ (OH). The active site extracted by heating and refluxing has strong V_ (OH) stretching vibration at 3300cm -1 or superposition vibration of v_ (NH) stretching vibration such as amino acid. The effective site extracted by percolation method is also strong here Absorption peak, and V_ (NH) at 3518cm ~ (-1) is more obvious. In addition, the total effective peak area of the infrared spectrum extracted by the two methods is only 45%, and the variation peak rate is as high as 83.8%. The infrared one-dimensional maps of different extraction sites all showed good stretching vibration of C = O, C-H, O-H, N-H and methyl and methylene groups. It was found that except the 745-715 cm -1 near the petroleum ether part, the number of peaks at the other positions was significantly less than that of the other three extraction parts. The number of peaks of the ethyl acetate part was larger and the peak shape was better. The chloroform and n- However, the number of peaks is similar but the peak shape is weak. According to the double index method, there are great differences between the chloroform and petroleum ether groups, while the chloroform and ethyl acetate fractions are similar.