论文部分内容阅读
目的 了解急性髓细胞白血病(acutemyeloidleukemia,AML)中胰岛素样生长因子2(insulinlikegrowthfactor2,IGF2)基因的印迹状态。方法 根据IGF2基因第9外显子具有Apa位点多态性,利用聚合酶链反应(PCR)结合限制性长度多态性技术,对26例AML患者骨髓细胞IGF2基因进行杂合子筛选,并进一步检测杂合子个体该基因的印迹丢失(lossofimprinting,LOI)。结果 11例AML患者为杂合子信息个体(1126),其中8例为双等位基因表达,即发生了LOI(811)。结论 IGF2基因的印迹丢失可能参与急性髓细胞白血病的发生和发展。
Objective To understand the imprinting status of insulin like growth factor 2 (IGF2) gene in acute myeloid leukemia (AML). Methods According to the Apa polymorphism of exon 9 of IGF2 gene, 26 cases of AML patients were screened for heterozygous IGF2 gene in bone marrow cells by polymerase chain reaction (PCR) and restriction fragment length polymorphism Heterozygous individuals were tested for loss of imprinting (LOI). Results Eleven patients with AML were heterozygous (1126) individuals, of whom 8 were allele-specific (ie, LOI (811)). Conclusion Impression loss of IGF2 gene may be involved in the occurrence and development of acute myeloid leukemia.