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目的 :观察再灌注损伤时脑组织Na+ -K+ -ATPase活性的改变及东莨菪碱对缺血再灌注脑组织Na+ -K+ -ATPase活性的影响。方法 :以选择性头部低温为阳性对照。通过低压低灌法造成完全性脑缺血模型。将 30只兔分为 :假手术组 (Ⅰ组 ) ,缺血组 (Ⅱ组 ) ,缺血再灌注组 (Ⅲ组 ) ,低温治疗组 (Ⅳ组 ) ,东莨菪碱治疗组 (Ⅴ组 ) ,东莨菪碱低温治疗组 (Ⅵ组 )。结果 :脑组织Na+ -K+ -ATPase活性以Ⅲ组最低 ;Ⅱ、Ⅳ、Ⅴ、Ⅵ组的Na+ -K+ -ATPase活性显著高于Ⅲ组 (P分别 <0 0 5或 <0 0 1) ,与Ⅰ组无显著差异 (P >0 0 5 )。Ⅳ、Ⅴ、Ⅵ 3组间的Na+ -K+ -ATPase活性无显著差异 (P >0 0 5 )。结论 :脑组织Na+ -K+ -ATPase对缺血损伤不敏感 ,对再灌注损伤敏感 ;东莨菪碱和低温一样 ,能保护缺血后再灌注脑组织Na+ -K+ -ATPase活性 ,但东莨菪碱和低温无明显的协同作用
Objective: To observe the changes of Na + -K + -ATPase activity in brain tissue during reperfusion injury and the effect of scopolamine on the activity of Na + -K + -ATPase in cerebral ischemia-reperfusion rats. Methods: Selective head hypothermia as a positive control. Complete cerebral ischemia model was created by hypoperfusion. Thirty rabbits were divided into sham operation group (group Ⅰ), ischemia group (group Ⅱ), ischemia reperfusion group (Ⅲ group), hypothermia treatment group (Ⅳ group), scopolamine treatment group (Ⅴ group), scopolamine Cryotherapy group (Ⅵ group). Results: The activity of Na + -K + -ATPase in brain tissue was the lowest in group Ⅲ. The activity of Na + -K + -ATPase in group Ⅱ, Ⅳ, Ⅴ and Ⅵ was significantly higher than that in group Ⅲ (P <0.05 or <0.01) There was no significant difference in group Ⅰ (P> 0.05). There was no significant difference in the activity of Na + -K + -ATPase among Ⅳ, Ⅴ and Ⅵ groups (P> 0.05). Conclusion: Na + -K + -ATPase in brain tissue is not sensitive to ischemia injury and is sensitive to reperfusion injury. Scopolamine can protect the activity of Na + -K + -ATPase in brain tissue after ischemia / reperfusion as well as hypothermia, but there is no significant difference between scopolamine and hypothermia Synergy