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目的研究动脉粥样硬化和冠脉气囊成形术后再狭窄中血管平滑肌细胞数量异常增多的机制，并初步探讨ＮＯ诱导血管平滑肌细胞凋亡的作用。材料和方法 以ＳＮＡＰ作为ＮＯ供体，采用ＨＥ，ＴＵＮＥＬ，荧光双染后光镜观察以及电镜、流式细胞术、细胞ＤＮＡ的琼脂糖凝胶电泳对ＳＮＡＰ作用下的细胞凋亡进行鉴定和检测。结果 观察发现， ０．４ｍｍｏｌ／Ｌ ＳＮＡＰ作用 ８－１０ ｈ即能明显地诱导培养的血管平滑肌细胞凋亡。结论 ＳＮＡＰ以浓度依赖方式诱导血管平滑肌细胞凋亡；细胞爬片的ＨＥ染色结合ＴＵＮＥＬ标记是检测凋亡的较为准确且简单的方法。
Objective To investigate the mechanism of abnormal increase of vascular smooth muscle cells in atherosclerosis and coronary artery stenosis after coronary balloon angioplasty and to explore the effect of NO on the apoptosis of vascular smooth muscle cells. MATERIALS AND METHODS SNAP was used as NO donor. The apoptosis of SNAP cells was identified and detected by light microscopy with HE, TUNEL, fluorescence double staining and electron microscopy, flow cytometry and DNA agarose gel electrophoresis. . The results showed that, 0.4mmol / L SNAP effect of 8-10 h can significantly induce apoptosis of cultured vascular smooth muscle cells. Conclusion SNAP induces vascular smooth muscle cell apoptosis in a concentration-dependent manner. Hematoxylin-eosin staining and TUNEL labeling are the most accurate and simple methods for detecting apoptosis.