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目的 研究何首乌水溶性成分 2 ,3,5 ,4′ 四羟基二苯乙烯 2 O β D葡糖苷 (STI)对溶血磷脂酰胆碱 (LPC)诱导人脐静脉内皮细胞株ECV30 4细胞中血管内皮生长因子 (VEGF)表达的影响。方法 在ECV30 4细胞培养基中加入LPC(2 5mg·L- 1 )或LPC与STI共孵 2 4h ,收集各组条件培养基 ,用基础酶联免疫吸附试验 (ELISA)检测各组条件培养基中VEGF蛋白含量 ;用原位杂交法、RT PCR及RealtimeRT PCR法检测LPC对内皮细胞VEGFmRNA的表达及STI的影响。结果 ECV30 4细胞暴露于LPC后 ,VEGF蛋白分泌明显增加 ;加入STI后VEGF蛋白含量明显降低 ;LPC可以使ECV30 4中VEGFmRNA的表达明显升高 ,并使VEGF1 6 5mRNA的表达升高 ;STI可剂量依赖性地抑制VEGF1 6 5mRNA的高表达。结论 LPC能诱导ECV30 4细胞表达高水平的VEGF蛋白及VEGFmRNA ,1× 1 0 - 5mol·L- 1STI可抑制LPC的作用 ,降低VEGF的高表达。
Objective To study the effect of water soluble component of Polygonum multiflorum 2, 3, 5, 4′-tetrahydroxystilbene 2 O β D glucoside (STI) on lysophosphatidylcholine (LPC)-induced vascular endothelial cells in human umbilical vein endothelial cell line ECV304 cells. The effect of growth factor (VEGF) expression. Methods LPC (25 mg·L-1) was added to ECV30 4 cell culture medium or LPC was co-incubated with STI for 24 hours. The conditioned medium of each group was collected and the conditioned medium of each group was detected by ELISA. The VEGF protein content was detected by in situ hybridization, RT PCR, and Realtime RT PCR to examine the effect of LPC on VEGF mRNA expression and STI in endothelial cells. Results After exposure to LPC, the secretion of VEGF protein was significantly increased in ECV304 cells; VEGF protein content decreased significantly after adding STI; LPC could significantly increase the expression of VEGF mRNA in ECV304 and increase the expression of VEGF165 mRNA; Dependently inhibits the high expression of VEGF165 mRNA. Conclusion LPC can induce high levels of VEGF protein and VEGF mRNA in ECV304 cells. 1×10 -5 mol·L- 1STI can inhibit the action of LPC and reduce the high expression of VEGF.