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目的揭示抗生素诱变的球形幽门螺杆菌（Ｈｐ）与定居有关毒力的变异情况。方法采用亚抑菌浓度抗生素作用，使５株Ｈｐ发生球形变异，检测球形Ｈｐ的尿素酶活性及其对Ｈｅｐ２细胞（喉癌上皮细胞）的粘附性，并用ＰＣＲ法检测其尿素酶Ａ，尿素酶Ｂ及粘附素基因。结果球形Ｈｐ尿素酶活性降低，对Ｈｅｐ２细胞的粘附性降低，电镜下可见球形Ｈｐ侵入细胞内。球形Ｈｐ的４１１ｂｐ尿素酶Ａ，１１５ｂｐ尿素酶Ｂ及３７５ｂｐ粘附素基因的ＰＣＲ均阳性。结论抗生素诱变的球形Ｈｐ与定居相关的毒力减弱，但其有关毒力基因片段未缺失。
Objective To reveal the variation of virulence of H. pylori (H. pylori) mutated by antibiotics. Methods The sub-bacteriostatic concentrations of antibiotics, the five strains of Hp globular mutation, detection of spherical Hp urease activity and Hep 2 cells (laryngeal epithelial cells) adhesion, and PCR assay urease A , Urease B and adhesin genes. Results spherical Hp urease activity decreased Hep 2 cell adhesion decreased, under the microscope, spherical Hp invaded the cell. Spherical Hp 411bp urease A, 115bp urease B and 375bp adhesin gene PCR were positive. Conclusion The antibiotic-induced globular Hp attenuated the colonization-related virulence, but its virulence-related gene fragments were not deleted.