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目的 建立显齿蛇葡萄片中二氢杨梅素含量的HPLC测定方法.方法 采用HPLC法对显齿蛇葡萄片中二氢杨梅素进行含量测定.选用Zorbax-SB C18色谱柱(4.6 mm× 250 mm,5μm);以甲醇0.05％磷酸水溶液(35:65)为流动相;检测波长:289 nm;流速:1.0 ml·min1;柱温:30℃.结果 二氢杨梅素在10.2～102.1 μg ·ml-1的范围内有良好的线性关系(r=0.9998),平均回收率97.6％,RSD为0.87％.结论 该方法简便、准确,重复性好,精密度高,可用于显齿蛇葡萄片中二氢杨梅素的含量测定.“,”Objective To establish an HPLC method for the determination of dihydromyricetin in ampelopis grossedentata tablets.Methods High performance liquid chromatography was employed to determine the content of dihydromyricetin.The mobile phase was a mixture of methonal and 0.05％ phosphoric acid (35 ∶ 65).The flow rate was 1.0 ·min 1 and the detection wavelength was 289 nm.Results The linear relationship was good within the concentration range of 10.2-102.1 μg · 1 (r =0.9998).The average recovery was 97.6％ (RSD=0.87％).Conclusion This method is accurate and simple with good specificity and reproducibility.It can be used in the content determination of ampelopis grossedentata tablets.