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近年来,国外某些学者认为不明原因不育妇女体内有抗卵抗体存在的可能性。实验证明卵子外层透明带物质具有强烈抗原性,而且证实哺乳动物卵子透明带和人的透明带间具有交叉免疫反应。本文用猪卵子间接荧光法研究妇女血清中抗透明带抗体存在的可能性。 材料和方法 一、检测用猪卵子的制备:刚成熟猪卵巢5g,剪碎后加胶原酶溶液15ml(1mg/ml),37℃酶解2小时。酶解液分别经200μ,150μ和70μ尼龙筛过滤,卵子持留在70μ尼龙筛布上,洗脱备用。
In recent years, some foreign scholars believe that unexplained infertility women have the possibility of anti-egg antibodies in vivo. Experiments show that the outer zona pellucida material has strong antigenicity, and confirmed that the mammalian zona pellucida and human zona pellucida have a cross-immune response. In this paper, pig egg indirect fluorescence method to study the possibility of anti-zona pellucida antibodies in the serum of women. Materials and methods First, the detection of pig egg preparation: just mature pig ovary 5g, after the addition of collagenase shearing 15ml (1mg / ml), 37 ° C for 2 hours. Hydrolyzate was filtered through 200μ, 150μ and 70μ nylon sieve, eggs remain in 70μ nylon mesh cloth, elution standby.