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目的运用基因芯片技术分析亚慢性汞中毒大鼠肾脏基因表达谱的变化。方法健康雄性SD大鼠20只随机分成染毒组和对照组,每组10只。染毒组皮下注射0.2 mg/kg氯化汞溶液2个月,建立亚慢性汞中毒肾脏损伤大鼠模型,用基因芯片方法筛选大鼠肾脏差异表达基因,并用逆转录-聚合酶链反应(RT-PCR)方法验证部分差异表达基因。结果筛选出亚慢性汞中毒肾脏差异表达基因385个,其中上调139个,下调246个。上调基因主要涉及毒物异物应答、炎症应答、神经递质代谢、溶质体转运等功能;下调基因主要涉及基础物质代谢、细胞信号传导、免疫调节、细胞增殖、转录调节等功能基因。RT-PCR方法验证结果与芯片结果一致。结论汞中毒肾脏损伤是多基因参与的结果;筛选出的明显差异表达基因可能在肾脏损伤机制中发挥重要作用,可能是氯化汞肾脏毒性作用的敏感基因或靶基因。
Objective To analyze the changes of gene expression profiles in kidney of sub-chronic mercury poisoning rats using gene chip technique. Methods Twenty healthy male Sprague-Dawley rats were randomly divided into treatment group and control group, with 10 rats in each group. The rats in the exposure group were injected subcutaneously with 0.2 mg / kg mercuric chloride solution for 2 months to establish a rat model of renal damage induced by subchronic mercury poisoning. The differentially expressed genes in kidney of rats were screened by gene chip and analyzed by reverse transcription - polymerase chain reaction -PCR) method to validate some differentially expressed genes. Results A total of 385 genes were differentially expressed in kidney of sub-chronic mercury poisoning, of which 139 were up-regulated and 246 were down-regulated. Up-regulated genes are mainly involved in foreign body response, inflammatory response, neurotransmitter metabolism, solute transport and other functions; down-regulated genes are mainly involved in basic material metabolism, cell signaling, immune regulation, cell proliferation, transcriptional regulation and other functional genes. RT-PCR method to verify the results and chip results. Conclusions Mercury poisoning is a result of multiple genes involved in kidney injury. The differentially expressed genes selected may play an important role in the mechanism of renal injury, which may be the sensitive gene or target gene of mercury chloride toxicity.