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本研究以云南2009-48和广东2010-13为实验材料,通过PCR方法扩增克隆了2种不同的白茅的45S rDNA IGS。序列分析结果表明:云南2009-48的45S rDNA IGS序列长度为2 613 bp,GC含量为64.6%;广东2010-13的45S rDNA IGS序列长度为2 882 bp,GC含量64.4%;它们的IGS序列一致性为84.5%。45S rDNA IGS均由NTS和ETS组成:云南2009-48的NTS和ETS长度分别为1 441 bp和1 172 bp,GC含量分别为63.5%和66.1%;广东2010-13的NTS和ETS长度分别为1 719 bp和1 163 bp,GC含量分别为64.2%和65.3%;它们的NTS序列一致性为76.5%,而ETS的NTS序列一致性高达96.2%。NTS中存在4种类型的亚重复序列(SR1,SR2,SR3,SR4),ETS中存在1种类型的亚重复序列(SR5),序列一致性为74.0%~93.0%。通过软件辅助分析以及与其他植物的比较分析,我们发现了预测的转录起始位点和转录终止位点,并且也存在着大量的甲基化位点,它们在调控rRNA转录方面起着非常重要的作用。此外,聚类分析结果表明白茅与甘蔗的亲缘关系相对较近,为拓宽甘蔗遗传基础提供了理论依据。
In this study, Yunnan 2009-48 and Guangdong 2010-13 were used as experimental materials to amplify and clone 45S rDNA IGS from two different species of Pseudostellariae Setaria by PCR method. The results of sequence analysis showed that the 45S rDNA IGS sequence of Yunnan 2009-48 was 2 613 bp in length with a GC content of 64.6%. The length of 45S rDNA IGS sequence was 2 882 bp in Guangdong 2010-13 with a GC content of 64.4% Consistency is 84.5%. The 45S rDNA IGS consisted of NTS and ETS. The NTS and ETS lengths of Yunnan 2009-48 were 1 441 bp and 1 172 bp, respectively, with GC contents of 63.5% and 66.1% respectively. The lengths of NTS and ETS in Guangdong 2010-13 were 1 719 bp and 1 163 bp with GC contents of 64.2% and 65.3%, respectively. Their NTS sequence identity was 76.5%, while the NTS sequence identity of ETS was as high as 96.2%. There are 4 types of sub-repeats (SR1, SR2, SR3, SR4) in NTS. There are 1 types of sub-repeats (SR5) in ETS, with sequence identities of 74.0% -93.0%. Through software-assisted analysis and comparative analysis with other plants, we found predicted transcription initiation sites and transcription termination sites, and also a large number of methylation sites, which play a very important role in the regulation of rRNA transcription Role. In addition, the results of cluster analysis showed that the genetic relationship between P. solanacearum and sugarcane was relatively close, which provided a theoretical basis for broadening the genetic basis of sugarcane.