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本研究筛选出油松-褐环乳牛肝菌菌根根际土中高效解磷钾细菌并对其进行种类鉴定。采用平板稀释法自菌根际土中筛选解磷钾细菌,并用钼蓝比色法和原子吸收法分别对其液体培养5 d后的菌株进行解磷能力和解钾能力测定,筛选出高效的解磷钾细菌。同时,对筛选出的菌株进行显微观察、生物学鉴定和16Sr DNA序列分析。通过筛选得出解有机磷细菌P6和解无机磷细菌P15能力较强,其培养液中有效磷浓度分别为283 mg/L和898.5 mg/L,经鉴定P6属于节杆菌属(Arthrobacter sp.),P15为荧光假单胞菌(Pseudomonas fluorescens),以及2株能力较强的解钾细菌K1和K12,其培养液中有效钾浓度分别为21.6 mg/L,19.3 mg/L,经鉴定K1为壤霉菌属(Agromyces cerinus),K12为中华根瘤菌属(Sinorhizobium sp.)。由此可见,油松-褐环乳牛肝菌菌根根际存在高效解磷钾细菌,褐环乳牛肝菌可以通过影响根际解磷钾细菌的种类及能力来改善油松幼苗的根际磷钾营养状况。
This study screened Pinus tabulaeformis - brown ring lactobacillus mycorrhizal rhizosphere soil high efficiency phosphorus and potassium bacteria and its species identification. Phosphate solubilizing potassium bacteria were screened from the rhizosphere soil by using plate dilution method. The bacteria strains that were cultured for 5 d in liquid culture were determined by molybdenum blue colorimetric method and atomic absorption spectrometry, respectively. Phosphorus and potassium bacteria. Meanwhile, the selected strains were observed microscopically, biological identification and 16Sr DNA sequence analysis. The results showed that P6 had strong ability to hydrolyze P6 and P15, and the available P concentrations in the culture medium were 283 mg / L and 898.5 mg / L, respectively. It was identified that P6 belonged to Arthrobacter sp. P15 was Pseudomonas fluorescens and two K-solubilizing bacteria, K1 and K12, with effective potassium concentrations of 21.6 mg / L and 19.3 mg / L, respectively, and K1 was identified as soil Agromyces cerinus, K12 is Sinorhizobium sp. It can be seen from the above results that there are highly efficient P and K-solubilizing bacteria in the rhizosphere of B. tabulaeformis and B. canadensis, and P. rhynchophylla can improve Rhizosphere Phosphorus Potassium nutrition status.