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[目的]了解汶川大地震后某板房社区内流行性脑膜炎奈瑟菌(Neisseria meningitides,Nm)带菌率状况和主要流行的血清群,为评价灾区卫生防疫效果和调整卫生防疫措施提供依据。[方法]对灾区某板房社区人群进行单纯随机抽样,采集咽拭子样品107份,采用传统的细菌学分离培养方法和分子生物学Taqman-MGB探针RT-PCR技术对流脑带菌率进行检测;对分离所得菌株进行血清学鉴定和PCR方法分群。[结果]对所采集的107份样品采用分子生物学Taqman-MGB探针RT-PCR技术检出3例阳性;细菌学分离培养得到3株阳性菌株,两种检测方法阳性样品编号一致,检测结果吻合,阳性率为2.80%。3株阳性菌株经血清学和分子生物学PCR方法分群,分别为B群Nm2株,C群Nm1株。[结论]与四川省流脑检测点及全国其他地区流脑带菌水平相比,社区流脑带菌率没有明显差别,流行仍以B群为主,但是流脑C群的出现应该引起足够的重视,防止流脑疫情的出现。
[Objective] To understand the prevalence of serogroups of epidemic Neisseria meningitides (Nm) and the main epidemic serogroups in a boarding community after Wenchuan Earthquake, and provide the basis for evaluating the epidemic prevention and epidemic prevention measures and adjusting the epidemic prevention measures. [Method] A total of 107 samples of throat swabs were collected from a community in a disaster area with random sampling. Samples were collected by traditional bacteriological isolation and culture methods and molecular Taqman-MGB probe RT-PCR The isolates were isolated by serological method and PCR method. [Results] Three of the 107 samples collected were positive by molecular biological Taqman-MGB probe RT-PCR. Three positive strains were obtained by bacteriological isolation and culture. The positive samples of the two methods were consistent. The results of the detection Anastomosis, the positive rate was 2.80%. Three strains of positive strains were divided by serological and molecular biological PCR methods, respectively B group Nm2 strain, C group Nm1 strain. [Conclusion] Compared with the meningitis detection points in Sichuan province and the levels of meningococcal infection in other parts of the country, there is no significant difference in the prevalence of meningococcal infection in community and the epidemic is still dominated by group B. However, the occurrence of meningococcal C group should pay enough attention , To prevent the emergence of meningitis.