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为在分子水平上探索铝是否影响RARα受体调节α-ADAM10分泌酶的机制研究。研究人员取40只健康雄性SD大鼠,按体重随机分为4组:溶剂对照(生理盐水)、低剂量(Al3+0.4 mg/kg·d)、中剂量(Al3+0.8 mg/kg·d)和高剂量组(Al3+1.2 mg/kg·d),腹腔注射染毒,连续5 d,休息2 d,共2个月。处死大鼠,分别取大脑皮质和海马,保存于-80℃。采用石墨炉原子吸收法检测各组铝含量,蛋白免疫印迹法检测RARα受体和α-ADAM10分泌酶的表达。随
To explore the molecular level of aluminum whether the impact of RARα receptor regulation of α-ADAM10 secretase mechanism. Forty healthy male Sprague-Dawley rats were randomly divided into four groups according to body weight: saline control, low dose (Al3 + 0.4 mg / kg · d), middle dose (Al3 + 0.8 mg / kg · d ) And high-dose group (Al3 + 1.2 mg / kg · d). The mice were injected intraperitoneally for 5 days and rested for 2 days for 2 months. The rats were sacrificed and the cerebral cortex and hippocampus were removed and stored at -80 ° C. The contents of aluminum in each group were determined by graphite furnace atomic absorption spectrometry. The expressions of RARα receptor and α-ADAM10 secretase were detected by Western blotting. With