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药物的遗传毒性是药物学中的一个重要课题。它可在人群中留下某些潜在危害因素,是对人类健康的一种挑战。近年来已建立许多测定细胞遗传损伤的短期试验。微核试验(Micro-nucleus Test)就是其中之一。按微核形成的原理为,一切进行分裂的细胞,在染色体断裂剂(Clastogens)或能影响纺锤体的毒物(Spindlepoisons)的作用下均能产生微核。故此试验可测定药物对染色体的损伤作用。1970年Schmid和Heddle 分别推荐用骨髓微核方法代替麻烦、费时的骨髓中期细胞染色体分析法,并提出有
Genotoxicity of drugs is an important topic in pharmacology. It leaves some potential hazards in the population and is a challenge to human health. In recent years, many short-term tests have been established to determine cytogenetic damage. Micro-nucleus Test is one of them. Micronuclei are based on the principle that all dividing cells can produce micronuclei under the action of Clastogens or Spindlepoisons, which can affect the spindle. Therefore, the test can determine the role of drugs on chromosome damage. In 1970, Schmid and Heddle recommended the use of bone marrow micronucleus method instead of cumbersome, time-consuming metaphase cell chromosome analysis, and proposed