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发锌含量能较敏感地反映个体较长时间内锌的积累状况和营养水平.为了解儿童体内含锌量水平,我们于1999年3月对7岁以下儿童进行了发锌检测.1 对象与方法1.1 对象 将牟平区4所幼儿园7岁以下儿童按年龄0～、1～、3～分成3组,每组按10%的比例随机分别抽取37、46、49名,共计112名.其中男66名,女46名,性别比例均衡.1.2 方法 采集受检儿童后枕部头发约1g,将发样用湿热的5%中性洗涤剂(上海产海鸥牌洗涤剂),浸泡30min,倒入自制清洗器内,流水冲洗干净,蒸馏水洗3次,重蒸馏水洗1次,80～90℃干燥2～4h.精确称取发样(精确至0.001g),用硝酸湿法消解后定容.测定仪器为北京瑞利分析仪器公司产的WFX-ID型原子吸收分光光度计,锌元素用火焰法测定.1.3 判断标准 发锌值≥90μg为正常,<70μg/g为绝对缺锌,70～90μg/g为边缘缺锌.2 结果2.1 不同年龄、性别儿童发锌含量 结果见表1.其中男性66例,发锌平均含量87.58μg/g,女性46例,发锌平均含量86.48μg/g.男女发锌含量无差异(t=0.3041,P>0.05).
Hair zinc content can more sensitively reflect the individual zinc accumulation over a long period of time and nutrition levels in order to understand the level of children’s zinc content, we conducted in March 1999 for children under the age of 7 were zinc detection.1 objects and Methods 1.1 Subjects The children under the age of 7 in 4 kindergartens in Muping District were divided into 3 groups according to their ages 0 ~, 1 ~, 3 ~, and 37,46,49 were randomly selected from each group according to the proportion of 10%, a total of 112. Among them, male 66, female 46, the proportion of gender balance .1.2 Methods After the collection of children with occipital hair about 1g, hair samples will be with 5% neutral heat neutral detergent (Shanghai Seagull brand detergent), soak 30min, into Self-made cleaner, running water rinse, distilled water washed 3 times, double distilled water washed 1, 80 ~ 90 ℃ drying 2 ~ 4h. Accurately weigh the hair sample (accurate to 0.001g), with nitric acid wet digestion volume. The measuring instrument is WFX-ID atomic absorption spectrophotometer produced by Beijing Rayleigh Analytical Instrument Co., Ltd. The zinc element is determined by the flame method.1.3 Judgment Standard The hair zinc value ≥90μg is normal, <70μg / g is absolute zinc deficiency, 70 ~ 90μg / g as the edge of zinc deficiency.2 results 2.1 different age, gender, children hair zinc content results in Table 1. There were 66 males , The average content of hair zinc was 87.58μg / g, the number of females was 46, the average content of hair zinc was 86.48μg / g, there was no difference between male and female hair zinc content (t = 0.3041, P> 0.05).