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通过RT-PCR,RACE等技术克隆得到中华绒螯蟹卵黄蛋白原的cDNA序列全长,其长度为7 921bp,软件分析后知其含有一个7 689 bp的开放阅读框,其5’和3’的非翻译区分别为28 bp和204 bp;预测该序列编码一个含2 562个氨基酸残基的蛋白质,和其它已知卵黄蛋白原序列的蟹类相比,理化性质、空间构型相似,且含有2个结构域:参与脂质转运的卵黄蛋白原N端结构域(Vitellogenin_N),以及血管性血友病因子(von Willebrand factor)D型结构域(VWD)。将其开放阅读框翻译为氨基酸序列后进行数据库对比(BLASTP),与其他已知的甲壳类卵黄蛋白原序列间存在33%～77%的相似性。
The full-length cDNA sequence of yolk protein from C. sinensis was cloned by RT-PCR, RACE and so on. The length of the cDNA was 7 921 bp. After analysis by software, it contained a 7 689 bp open reading frame with 5 ’and 3’ Of the untranslated region were 28 bp and 204 bp, respectively. It was predicted that this sequence encodes a protein with 2 562 amino acid residues. Compared with other known cynostenoid sequences, the physical and chemical properties and spatial configuration are similar Contains 2 domains: Vitellogenin_N involved in lipid transport, and von Willebrand factor D domain (VWD). The BLASTP was translated after translating the open reading frame (ORF) of the open reading frame (ORF) into amino acid sequence, with 33% ~ 77% similarity with other known crustacean vitellogenin sequences.