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目的:探讨不同培养条件对宫颈鳞癌原代培养的可行性。方法:取确诊为宫颈鳞癌患者的活检或手术标本,通过对消化时间及重悬液量进行改良原代细胞培养,观察鳞癌细胞生长状况,并比较其与传统消化法的细胞生长率,免疫组化进行细胞鉴定。结果:消化时间为3小时,重悬液量为1ml时细胞生长率最高。改良法24小时细胞生长率为61.54%,传统消化法24小时细胞生长率为7.69%(x2=5.14,P<0.05)。活检标本细胞生长率80%,手术标本3例无一例细胞生长。结论:采用消化时间为3小时,重悬液量为1 ml成功建立了宫颈鳞癌原代培养方法,活检标本较手术标本更易培养成功。
Objective: To investigate the feasibility of primary culture of cervical squamous cell carcinoma under different culture conditions. Methods: The biopsies or surgical specimens of patients diagnosed as squamous cell carcinoma of the cervix were collected. The primary cells were cultured for the purpose of improving the digestion time and resuspension volume. The growth of squamous cell carcinoma was observed and compared with the traditional cell culture, Immunohistochemistry for cell identification. Results: The digestion time was 3 hours, and the cell growth rate was the highest when resuspension volume was 1ml. The improved cell growth rate was 61.54% at 24 hours. The cell growth rate at 24 hours was 7.69% (x2 = 5.14, P <0.05) by traditional digestion method. Biopsy specimens of cell growth rate of 80%, 3 cases of surgical specimens without cell growth. Conclusion: The primary culture method of cervical squamous cell carcinoma was successfully established with 3 hours digestion time and 1 ml resuspension volume. Biopsy specimens were more easily cultured than surgical specimens.