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目的探讨TLR4基因3’未翻译区(3’UTR)11367位点的多态性在儿童哮喘人群中的分布及其与患儿特应质、肺炎支原体感染、过敏原和肺功能参数的关系。方法本研究纳入哮喘患儿222例,正常对照290例。采用单管双向等位基因特异性扩增技术(SB-ASA)检测TLR4基因3’UTR 11367位点的基因多态性。使用被动凝集法检测血清肺炎支原体抗体滴度。通过皮肤点刺试验(Skin prick test,SPT)检测常见过敏原。结果哮喘组中TLR4 G11367C位点基因型GG、GC/CC的频率分别是89.6%和10.4%,等位基因G、C的频率分别是93.8%和6.2%,与对照组相比差异无统计学意义(P>0.05);相较于GC型,GG型的哮喘患儿湿疹史更高(P=0.024,OR=2.957,95%CI为1.117～7.828),屋尘螨过敏程度也较GC型重(P=0.021);GG型和GC型患儿肺功能水平没有显著性差异。结论 TLR4基因3’UTR基因多态性与儿童哮喘易感性不相关,但与哮喘患儿有无湿疹史及屋尘螨的过敏程度相关。
Objective To investigate the distribution of 11367 polymorphism in 3 ’untranslated region (3’UTR) of TLR4 gene in children with asthma and its relationship with atopy, Mycoplasma pneumoniae infection, allergen and pulmonary function parameters. Methods This study included 222 children with asthma and 290 normal controls. Single-tube bi-directional allele-specific amplification (SB-ASA) was used to detect the gene polymorphism of 3’UTR 11367 in TLR4 gene. The use of passive agglutination test serum Mycoplasma pneumoniae antibody titers. Common allergens were detected by Skin prick test (SPT). Results The frequencies of GG and GC / CC genotypes of TLR4 G11367C in asthma group were 89.6% and 10.4%, respectively. The frequencies of allele G and C were 93.8% and 6.2%, respectively, with no significant difference compared with control group (P = 0.024, OR = 2.957, 95% CI: 1.117-7.828), and the allergic degree of house dust mite was also higher than that of GC type (P = 0.021). There was no significant difference in pulmonary function between children with GG and GC genotypes. Conclusion The polymorphism of 3’UTR gene in TLR4 gene is not related to susceptibility to childhood asthma, but it is related to the history of eczema in children with asthma and the allergy to house dust mite.