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目的:观察自体骨髓干细胞移植治疗糖尿病足的临床疗效。方法:应用自体骨髓干细胞移植治疗64例糖尿病足患者,采集自体骨髓200～250ml,用淋巴细胞分离液以梯度离心法分离,配成单个核细胞悬浊液约50ml供移植用,计数单个核细胞总数为(4～8)×108个/ml,行下肢缺血肌肉局部注射。结果:大部分患者肢体疼痛缓解,冷感、间歇性跛行均有不同程度的改善,12周46例溃疡明显好转,踝肱压指数35例改善。结论:自体骨髓干细胞移植治疗糖尿病足是一种简单、安全、有效的方法,能提高患者生活质量。
Objective: To observe the clinical efficacy of autologous bone marrow stem cell transplantation in the treatment of diabetic foot. Methods: Sixty-four patients with diabetic foot were treated with autologous bone marrow stem cell transplantation. The autologous bone marrow was collected from 200 to 250ml and separated by gradient centrifugation using lymphocyte separation fluid. The single cell suspension was dubbed for about 50ml for transplantation. The number of mononuclear cells The total number of (4 ~ 8) × 108 / ml, the lower limb ischemia muscle local injection. Results: Most patients’ limb pain relief, cold sensation and intermittent claudication had different degrees of improvement. In the 12 weeks, 46 cases of ulcer obviously improved and 35 cases of ankle-brachial pressure index improved. Conclusion: Autologous bone marrow stem cell transplantation for the treatment of diabetic foot is a simple, safe and effective method to improve the quality of life of patients.