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目的:探讨骨髓间充质干细胞(BM-MSCs)在正常小鼠体内从皮下向不同器官迁移的能力。方法:从β半乳糖苷酶(β-gal+)转基因小鼠分离骨髓细胞,经贴壁和传代培养获得第3代β-gal+BM-MSCs,皮下注射给正常6周龄C57BL/6J野生型小鼠。8周后通过LacZ染色示踪β-gal+BM-MSCs在受体小鼠不同组织器官的分布,并对供体第3代β-gal+BM-MSCs基质金属蛋白酶(MMPs)的基因表达水平进行检测。结果:β-gal转基因小鼠来源的供体第3代BM-MSCs呈现LacZ染色阳性。第3代的β-gal阳性BM-MSCs皮下注射8周后,在受体小鼠肺、脑、心、肝、肾内检测到LacZ染色阳性的细胞。第3代β-gal+BM-MSCs表达MMPs基因。结论:皮下注射的BM-MSCs能够在正常小鼠体内经血液循环迁移到不同器官,并可能分化成为不同器官的终末分化细胞,参与正常组织的自我更新。
Objective: To investigate the ability of bone marrow mesenchymal stem cells (BM-MSCs) to migrate from subcutaneous to different organs in normal mice. METHODS: Bone marrow cells were isolated from β-galactosidase (β-gal +) transgenic mice and the 3rd generation β-gal + BM-MSCs were obtained by adherent and subculture. The normal 6-week old C57BL / 6J wild type Mouse. After 8 weeks, the distribution of β-gal and BM-MSCs in different tissues and organs of recipient mice was observed by LacZ staining. The gene expression levels of matrix metalloproteinases (MMPs) in the third generation of β-gal and BM- Test. Results: The third generation of BM-MSCs derived from β-gal transgenic mice showed positive LacZ staining. After 3 weeks of subcutaneous injection of β-gal positive BM-MSCs, lacZ positive cells were detected in lung, brain, heart, liver and kidney of recipient mice. The third generation of β-gal + BM-MSCs express the MMPs gene. CONCLUSION: Subcutaneously injected BM-MSCs can migrate to different organs through blood circulation in normal mice and may differentiate into terminal differentiated cells of different organs and participate in the self-renewal of normal tissues.