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目的观察医院分离的2株耐甲氧西林金黄色葡萄球菌(MRSA)和金黄色葡萄球菌标准株(ATCC 6538)对6种消毒剂的抗性与耐药基因变化。方法选择最小抑菌浓度(MIC)试验、悬液定量杀菌试验和耐消毒剂基因PCR检测。结果 MIC检测,医院分离的2株MRSA,对6种消毒剂的MIC,明显高于标准菌株。只有醋酸氯己定对MRSA-1的MIC值与标准菌株相同。PCR检测,2株MRSA均未携带耐消毒剂基因qacA/B。定量杀菌实验,苯扎氯铵、过氧乙酸、双癸基二甲基氯化铵对MRSA-1菌株的杀灭对数值低于标准菌株;苯扎氯铵、过氧乙酸、有效氯对MRSA-2菌株的杀灭对数值低于标准株。结论医院分离的2株MRSA对消毒剂的抗性普遍高于标准菌株,PCR检测未发现qacA/B基因。
Objective To observe the changes of resistance and resistance genes of two strains of methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus (ATCC 6538) isolated from hospitals. Methods Select the minimum inhibitory concentration (MIC) test, quantitative bactericidal test suspension and resistance to disinfectant gene PCR test. Results MIC test, the two MRSA isolates from the hospital, the MIC of 6 kinds of disinfectants was significantly higher than the standard strains. Only MIC of chlorhexidine acetate against MRSA-1 was the same as the standard strain. PCR detection, two strains of MRSA did not carry resistant gene qacA / B. Quantitative sterilization experiments, benzalkonium chloride, peracetic acid, didecyl dimethyl ammonium chloride kill MRSA-1 strain strains lower than the standard strain; benzalkonium chloride, peracetic acid, available chlorine on MRSA -2 strains kill logarithmically lower than the standard strain. Conclusion The resistance of two strains of MRSA isolated from hospitals is generally higher than that of the standard strains. The qacA / B gene was not detected by PCR.