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目的:研究毒蜥毒素中Helokinestatin的4种亚型对大鼠尾动脉平滑肌的毒性作用。方法:取成年雄性大鼠尾动脉,分离得到平滑肌肌束。将动脉平滑肌标本连到张力换能器上,分别以不同浓度的bradykinin(缓激肽)和bradykinin、Helokinestatin联合作用于大鼠平滑肌,统计各个浓度的肌肉收缩张力的相对值作为纵坐标,以多肽浓度的-log值作为横坐标进行线性回归作图,进行统计学分析。观察4种Helokinestatin处理标本的肌张力改变情况。结果:与单纯给予bradykinin处理相比,同时给予bradykinin和Helokinestatin处理的标本,在多肽浓度为10~(-7)M~10~(-5)M的肌张力明显增加,差异有统计学意义(P<0.05)。结论:.4种Helokinestatin在多肽浓度为10~(-7)M~10~(-5)M时,都能拮抗缓激肽对大鼠平滑肌的舒张作用.并且这种拮抗作用是非竞争性的。
Objective: To study the toxic effects of four isoforms of Helokinestatin on rat tail artery smooth muscle. Methods: Tail artery was obtained from adult male rats, and muscle bundles of smooth muscle were isolated. The arterial smooth muscle specimens were connected to the tension transducer. The bradykinin (Bradykinin) and bradykinin (Helicastatin) were mixed with the smooth muscle of rat in different concentrations respectively. The relative values of muscle contractile tension at various concentrations were taken as the ordinate, Concentration-log value as the abscissa linear regression mapping, statistical analysis. The changes of muscle tone in 4 Helokinestatin treated groups were observed. RESULTS: Compared with the bradykinin treatment alone, the bradykinin and Helokinestatin administration significantly increased the muscle tone at the concentration of 10 -7 M 10 -5 M, the difference was statistically significant ( P <0.05). CONCLUSION: Four kinds of Helokinestatin can antagonize the relaxing effect of bradykinin on smooth muscle of rats at the concentration of 10 -7 M ~ 10 -5 M, and the antagonism is noncompetitive .