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目的:观察TLR2、TLR4在新生儿败血症病程中的变化,探讨其在新生儿抗感染免疫中的作用。方法:将60例不同胎龄(30~42周)新生儿分为败血症组和对照组。应用流式细胞仪检测败血症组感染后第1-2天、和非感染组第1-2天的外周血单核细胞表面TLR2、TLR4蛋白表达;同时检测血CRP、血小板。结果:感染后第1-2天,败血症组TLR2、TLR4百分含量在单核细胞的表达水平及CRP均高于非感染组(P均<0.05),两组治疗前后血小板均变化不明显。结论:新生儿败血症患儿外周血单个核细胞表面TLR2、TLR4蛋白表达异常增高,提TLR信号传导途径参与了新生儿的抗感染免疫机制,与新生儿败血症的发生、发展密切相关。
Objective: To observe the changes of TLR2 and TLR4 in the course of neonatal sepsis and explore the role of TLR2 and TLR4 in anti-infective immunity of neonates. Methods: Sixty newborns with different gestational age (30-42 weeks) were divided into sepsis group and control group. Flow cytometry was used to detect the expression of TLR2 and TLR4 on the peripheral blood mononuclear cells in sepsis group at day 1-2 after infection and in non-infected group at day 1-2. Blood CRP and platelets were also detected. Results: The levels of TLR2 and TLR4 in monocytes and CRP in sepsis group were significantly higher than those in non-infected group on day 1-2 after infection (all P <0.05). No significant changes were found in platelet count before and after treatment. CONCLUSIONS: The expression of TLR2 and TLR4 on peripheral blood mononuclear cells in neonates with sepsis is abnormally elevated. The TLR signaling pathway is involved in the anti-infection immune mechanism of neonates, which is closely related to the occurrence and development of neonatal sepsis.