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目的:观察正常、超重和肥胖3组人群皮下和大网膜脂肪组织中维生素D受体(vitamin D receptor,VDR)mRNA及其蛋白表达水平的差异。方法:用半定量RT-PCR方法检测20例正常组、35例超重组、27例肥胖组人群的腹部皮下和大网膜脂肪组织VDR基因的mRNA表达水平;用Western blot方法检测VDR的蛋白表达水平。结果:在皮下脂肪组织中,肥胖组VDRmRNA和蛋白表达水平明显低于正常组和超重组(P<0.05);在大网膜脂肪组织中,超重组VDR mRNA及其蛋白表达增高(P<0.05),肥胖组VDR mRNA及其蛋白表达与超重组相比降低(P<0.05)。结论:肥胖患者脂肪组织中VDR表达水平降低,可能是临床上补充维生素D干预肥胖达不到预期效果的机制之一。
OBJECTIVE: To observe the differences of vitamin D receptor (VDR) mRNA and protein expression between subcutaneous and omental adipose tissue in normal, overweight and obese three groups. Methods: The mRNA expressions of VDR gene in abdominal subcutaneous and omental adipose tissue were detected by semi-quantitative RT-PCR in 20 normal subjects, 35 over-weight and 27 obese subjects. The protein expression of VDR was detected by Western blot Level. Results: In subcutaneous adipose tissue, the expression of VDR mRNA and protein in obese group was significantly lower than that in normal group and overweight group (P <0.05). In omental adipose tissue, the expression of VDR mRNA and protein in overweight group was increased (P <0.05 ). The expression of VDR mRNA and protein in obese group was lower than that in overweight group (P <0.05). Conclusion: The decreased expression of VDR in adipose tissue of obese patients may be one of the mechanisms that vitamin D supplementation can not achieve the desired effect in clinic.