论文部分内容阅读
实验组大鼠腹腔1次注入D-半乳糖胺(600mg/kg)致成急性肝损伤;给予对照组大鼠等量生理盐水。于给药后1、2、3、5、7、10d,禁食12h后分批取胰尾,制石蜡切片。用免疫组织化学PAP法分别显示含胰岛素的B细胞,含高糖素的A细胞和含生长抑素的D细胞。对这些细胞的免疫反应性作半定量观察和分析,以判断肝损伤后胰岛细胞内分泌活动的变化。结果表明,给药后1～5d肝损伤严重,但肝再生也很活跃。此时B细胞胰岛素免疫反应物减少;A细胞高糖素免疫反应物含量明显下降,尤以2、3d最显著;但D细胞的生长抑素免疫反应性则增强。给药后7d肝损伤已基本恢复,3种胰岛细胞的免疫反应性与对照组近似。本研究结果提示,胰岛B细胞和A细胞分泌活动的变化,与肝损伤和肝再生过程密切相关。D细胞的变化可能反映它们对A细胞和B细胞内分泌活动有调控作用。
Rats in the experimental group were injected intraperitoneally with D-galactosamine (600 mg/kg) for acute liver injury; rats in the control group were given normal saline. On the 1st, 2nd, 3rd, 5th, 7th, and 10th days after administration, the tail of the pancreas was taken in batches after fasting for 12 hours to prepare paraffin sections. The insulin-containing B cells, A-cells containing high-glucagon, and D-cells containing somatostatin were respectively shown by immunohistochemical PAP. The semi-quantitative observation and analysis of the immunoreactivity of these cells were performed to judge the changes of endocrine activity of islet cells after liver injury. The results showed that liver damage was severe from 1 to 5 days after administration but liver regeneration was also active. At this time, the B-cell insulin immunoreactive substance decreased; the content of A-cell high-glucagon immunoreactive substances decreased significantly, especially at 2 and 3 days, but the somatostatin immunoreactivity of D-cells increased. At 7 days after the administration, liver damage was basically restored. The immunoreactivity of the three islet cells was similar to that of the control group. The results of this study suggest that changes in the secretion activity of islet B cells and A cells are closely related to the process of liver injury and liver regeneration. Changes in D cells may reflect their regulatory effects on the endocrine activity of A and B cells.