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】用重复序列探针MGR586与限制性内切酶EcoR1组合,分别分析了我国1980~1997年在17个省市146块稻田内外的186个不同水稻品种上采集的445个水稻分离菌株及25种不同禾本科植物和杂草上采集的108个非水稻分离菌株的限制性片段长度多态性(RFLPs),依其MGR-DNA指纹的相似率,结合病菌的致病性测定,将表现为48个不同致病型的553个菌株区分为56个谱系。结果表明,稻瘟菌有远距离传播的可能性,并在适宜的气候和人工接种条件下,某些禾本科植物和杂草上的草瘟菌与稻瘟菌可彼此互交。这为稻瘟病的流行和预测提供了新的思路。该文用生物测定和分子生物学相结合的方法,揭示了我国在不同空间和时间的稻瘟菌群体存在着明显的遗传多样性及其流行因素,并对今后的抗病育种及其利用策略进行了讨论。
】 Using repeated sequence probe MGR586 and restriction endonuclease EcoR1, 445 rice isolates and 25 kinds of isolates collected from 186 different rice cultivars in 146 paddy fields in 17 provinces and cities in 1980-1997 were analyzed. The restriction fragment length polymorphisms (RFLPs) of 108 non-rice isolates collected from different grasses and weeds were characterized by the similarity of MGR-DNA fingerprinting and the pathogenicity of pathogenic bacteria 553 strains of different pathogenicity were divided into 56 lineages. The results showed that there was a possibility that Magnaporthe grisea could spread long-rangely and that M. grisea and M. grisea could be crossed with each other on some grasses and weeds under the appropriate climate and artificial inoculation conditions. This provided a new way of thinking for the epidemic and prediction of blast disease. In this paper, bioassay and molecular biology are combined to reveal the obvious genetic diversity and epidemiological factors of Magnaporthe grisea populations in different space and time in China. The future breeding strategies and their utilization strategies Discussed.