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12例月经规则的子宫肌瘤患者(35～47岁),根据基础体温(BBT)、超声及血浆孕酮(P)水下确定排卵日,于排卵后3～6天行子宫切除术时取新鲜黄体。除掉间质及血管后粉碎之,制成悬液,计数稀释后进行培养。培养液分含及无10%胎牛血清两组。培养1天组培液内人绒毛膜促性腺激素(hCG)(6～100ng/ml);或人生长激素(hGH)(250～4000ng/ml);培养5天组则加 hCG50ng/ml 或/和hGH2 000ng/ml。两组皆设对照。每天更换培液测 P 水平。培养结束时重复细胞计数。麻醉前还取血测 P 水平。
12 cases of menstrual rules of uterine fibroids (35 to 47 years old), according to basal body temperature (BBT), ultrasound and plasma progesterone (P) to determine the date of ovulation, 3 to 6 days after ovulation hysterectomy Fresh yellow body. Remove the interstitial and blood vessels after smashing, made of suspension, counting diluted culture. Broth with and without 10% fetal bovine serum two groups. Cultured human chorionic gonadotropin (hCG) (6-100 ng / ml); or human growth hormone (hGH) (250-4000 ng / ml) for 1 day; cultured for 5 days added hCG 50 ng / ml or / And hGH2 000 ng / ml. Both sets of control. Change the test fluid daily P level. Repeat cell counting at the end of culture. Anesthesia before taking blood test P level.