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以土壤中分离得到的一株拮抗菌——长枝木霉Trichoderma longibrachiatum T6为研究对象,通过优化培养基配方及助剂类型的筛选,成功研制了长枝木霉T.longibrachiatum T6水分散粒剂,并测定了其贮存稳定性。结果表明:制备长枝木霉T6水分散粒剂的最佳配方(质量分数)为:羧甲基纤维素(紫外保护剂)0.5%,碳酸钙(稳定剂)4%,聚乙烯基吡咯烷酮(PVP,黏结剂)5%,可溶性淀粉(崩解剂)5%,凹凸棒土(载体)5%及十二烷基硫酸钠(润湿剂)5%。由此配方制备的水分散粒剂中孢子含量为5.6×10~8 cfu/g,悬浮率53%,p H值6.0,水分含量3.5%,湿润时间52 s,且在冷藏温度为(4±2)℃条件下的稳定性显著高于在室温(25℃)下的。各项检测结果均符合国家标准。
Trichoderma longibrachiatum T6, an antagonistic bacterium isolated from soil, was successfully used to study the growth of T. longibrachiatum T6 water dispersible granules , And its storage stability was measured. The results showed that the optimum formulation (mass fraction) of Trichoderma longibrachiatum T6 water dispersible granules was 0.5% of carboxymethylcellulose (UV protectant), 4% of calcium carbonate (stabilizer), polyvinylpyrrolidone PVP, binder) 5%, soluble starch (disintegrant) 5%, attapulgite (carrier) 5% and sodium lauryl sulfate (wetting agent) 5%. The spore content of the water dispersible granule prepared by this formula is 5.6 × 10 ~ 8 cfu / g, the suspension rate is 53%, the pH value is 6.0, the moisture content is 3.5%, the wetting time is 52 s, and the refrigerating temperature is (4 ± 2) ° C was significantly higher than at room temperature (25 ° C). The test results are in line with national standards.