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单倍体用于诱发突变和筛选试验的有利特点,是研究者能够用诱变剂诱发遗传变异并直接筛选理想突变体。本文研究 EMS 对烟草花药培养以及叶绿素含量和蛋白质变异的影响。材料和方法材料:从烟草品种 Wisconsin-38的花芽中获得花药。烟草植株在温室条件下生长(白天27℃,夜间24℃,16小时光照),栽在砂∶泥炭为1∶1的盆钵中,每7天用 Hoagland 氏2号溶液浇灌。方法:当花瓣长度等于萼片长度时采集花芽,用次氯酸钠(7％,V/V)表面消毒,随
A favorable feature of haploids used in mutation-inducing and screening assays is that researchers can induce genetic variation using mutagens and direct screening for ideal mutants. This article studies the effect of EMS on tobacco anther culture and chlorophyll content and protein variation. Materials and Methods Materials: Anther was obtained from flower buds of the tobacco variety Wisconsin-38. Tobacco plants were grown under greenhouse conditions (daytime 27 ° C, night 24 ° C, 16 hours light), planted in a bowl of sand: peat 1: 1, and irrigated with Hoagland’s No. 2 solution every 7 days. Methods: Flower buds were collected when the length of the petals was equal to the length of the sepals, surface disinfected with sodium hypochlorite (7%, V / V)