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Objective:To analyze the phytochemical composition and in vitro antioxidant properties of aqueous extract of Aerva Lanata(A.lanata) stem.Methods:During the preliminary phytochemical analysis,the aqueous extract of A.Ianata was screened for the presence of carbohydrates,proteins,phenolic compounds,oil and fats,saponins,flavonoids,alkaloids. tannins and phytosterols.Antioxidant activity of the extract was determined by 2.2-dipbenyl- 1-picrylhydrazyl radical scavenging activity,metal chelating activity,reducing power activity and DNA damage inhibition activity.Analysis of phenolic compounds was performed by FolinCiocaiteau reagent method and gradient high performance liquid chromatography technique. Results:Preliminary phytochemical analysis exhibited the presence of phenolic compounds, saponins.flavonoids.tannins and phytosterols as major phytochemical groups.The extract exhibited high 2.2—diphenyl-1-picrylhydrazyl radical scavenging activity(IC_(50)= 110.74μg/ mL).metal chelating activity(IC_(50)= 758.17μg/mL).reducing power activity and DIA damage inhibition efficiency.The extract was reported to possess a high amount of total phenolic content and some of them were identified as gallic acid(3,4,3-OH),apigenin-7—O-glucoside tapigetrin), quercetin-3—O-rutinoside(rutin) and myricetin(3,5,7,3,4,5-OH)by high performance liquid chromatography analysis.The extract was found non toxic towards human erythrocytes in the hemolytic assay(IC_(50)= 24.89 mg/mL).Conclusions:These results conclud that A.lanata stem possesses high antioxidant activity and can he used for the development of natural and sale antioxidant compounds.
Objective: To analyze the phytochemical composition and in vitro antioxidant properties of aqueous extract of Aerva Lanata (A. lanata) stem. Methods: During the preliminary phytochemical analysis, the aqueous extract of A. Ianata was screened for the presence of carbohydrates, proteins, tannins and phytosterols. Antioxidant activity of the extract was determined by 2.2-dipbenyl-1-picrylhydrazyl radical scavenging activity, metal chelating activity, reducing power activity and DNA damage inhibition activity. Analysis of phenolic compounds was performed by FolinCiocaiteau reagent method and gradient high performance liquid chromatography technique. Results: Preliminary phytochemical analysis exhibited the presence of phenolic compounds, saponins. flavonoids. tannins and phytosterols as major phytochemical groups. extract exhibited high 2.2-diphenyl-1 -picrylhydrazyl radical scavenging activity (IC_ (50) = 110.74 μg / mL) .metal chelating act ivity (IC 50 = 758.17 μg / mL) .reducing power activity and DIA damage inhibition efficiency efficiency. The extract was reported to possess a high amount of total phenolic content and some of them were identified as gallic acid (3,4,3 -OH), apigenin-7-O-glucoside tapigetrin, quercetin-3-O-rutinoside (rutin) and myricetin (3,5,7,3,4,5-OH) by high performance liquid chromatography analysis. was found non toxic towards human erythrocytes in the hemolytic assay (IC 50 (50) = 24.89 mg / mL) .Conclusions: These results conclud that A. lanata stem possesses high antioxidant activity and can he used for the development of natural and sale antioxidant compounds .