Determination of Araloside X in Aralia elata( Miq.) Seem. by HPLC

• 作 者: Yan LIU Bing HE Wei XIONG Chunhong LI Ji TIAN
• 机 构: Research Center for Drug and Functional Food,Luzhou Medical College,School of Public Health,Luzhou M
• 出 处: Medicinal Plant
• 发表日期: 2013年09期
• 关键词: Aralia acetonitrile oleanolic chromato purchased medicinal methanol stasis disti

[Objective]To detect the Araloside X content in Aralia elata(Miq.)Seem.by HPLC.[Methods]Chromatographic column was Kromasil C18column(250 mm×4.6 mm,5μm);mobile phase was acetonitrile-0.05%phosphoric acid solution(35∶65,V/V);detection wavelength was 203 nm;column temperature was 30℃;flow rate was 0.8 mL/min;and injection volume was 10μL.[Results]Araloside X showed good linear relationship with peak area(r=0.999 9)within the range of 0.60-4.80μg/mL.The average recovery rate was 98.14%and RSD was 1.13%.[Conclusions]This method was sensitive,accurate and repeatable,and could be used for the quality control of A.elata.

[Objective] To detect the Araloside X content in Aralia elata (Miq.) Seem. By HPLC. [Methods] Chromatographic column was Kromasil C18column (250 mm × 4.6 mm, 5 μm); mobile phase was acetonitrile-0.05% phosphoric acid solution 35:65, V / V); detection wavelength was 203 nm; column temperature was 30 ° C .; flow rate was 0.8 mL / min; and injection volume was 10 μL. [Results] Araloside X showed good linear relationship with peak area (r = 0.999 9) within the range of 0.60-4.80 μg / mL. The average recovery rate was 98.14% and RSD was 1.13%. [Conclusions] This method was sensitive, accurate and repeatable, and could be used for the quality control of A. elata.