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本文研究木犀草素的肝保护作用。以人肝癌细胞Hep G2为模型,以H_2O_2为氧化损伤来源,分为5个实验组包括空白组、损伤组、低浓度木犀草素保护组(0.5μM+300μM H_2O_2)、中浓度木犀草素保护组(5μM+300μM H_2O_2)和高浓度木犀草素保护组(10μM+300μM H_2O_2)测定细胞活力、活性氧(ROS)增长率、LDH、SOD和CAT酶活,测定细胞周期及细胞凋亡。经过10μM木犀草素保护,细胞活力提高到84%左右,ROS的增长率与空白组无显著性差异(p>0.05),LDH、SOD和CAT酶活力显著低于损伤组(p<0.05);流式细胞仪观察到随着木犀草素浓度增加,细胞的凋亡率减少并呈量效关系,而加药组的细胞周期与损伤组并无显著性差异(p>0.05)。木犀草素具有很好的肝保护能力,在食品、保健品和化妆品行业中具有潜在的应用价值。
This article studies the hepatoprotective effect of luteolin. Using human hepatoma Hep G2 cells as a model and H 2 O 2 as the source of oxidative damage, the experimental group was divided into five experimental groups including blank group, injury group, low concentration of luteolin protection group (0.5μM+300μM H 2 O 2 ), and medium concentration of luteolin. Cell viability, reactive oxygen species (ROS) growth rate, LDH, SOD, and CAT activity were measured in groups (5 μM + 300 μM H_2O_2) and high concentrations of luteolin (10 μM + 300 μM H_2O_2), and cell cycle and apoptosis were measured. After 10 μM luteolin protection, the cell viability increased to about 84%. The growth rate of ROS had no significant difference with the blank group (p>0.05). The LDH, SOD and CAT enzyme activities were significantly lower than the injury group (p<0.05). Flow cytometry observed that with the increase of luteolin concentration, the apoptotic rate of cells decreased and showed a dose-effect relationship, while there was no significant difference (p>0.05) between the cell cycle of the drug-adding group and the injury group. Luteolin has a good liver protection ability and has potential application value in the food, health care and cosmetics industries.