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目的 探讨生殖支原体（Ｍｇ）与肺部炎性疾病的关系。方法 应用原核生物通用引物ＰＣＲ直接测序克隆Ｍｇ１６ＳｒＲＮＡ基因片段序列，自行建立Ｍｇ 套式ＰＣＲ扩增法，检测健康儿童和肺部炎性疾病患儿咽拭子标本中的Ｍｇ－ ＤＮＡ。结果 健康儿童Ｍｇ 阳性率为５％ ，儿童肺炎、儿童支气管炎、新生儿肺炎者阳性率分别为２１．６％ 、２８．６％ 、２０％ （Ｐ均＜ ０．０１）。结论 Ｍｇ 可能与肺部炎性疾病相关联
Objective To investigate the relationship between mycoplasma genitalium (Mji) and inflammatory diseases of the lungs. Methods The sequence of Mg16SrRNA gene was directly cloned by PCR using universal primer of prokaryotic gene. Mg-DNA PCR was used to detect the Mg-DNA in throat swab specimens of healthy children and children with pulmonary inflammatory diseases. Results The positive rate of Mg in healthy children was 5%. The positive rates of pneumonia, bronchitis and neonatal pneumonia in children were 21.6%, 28.6% and 20% respectively (all P <0.01). Conclusion Mg may be associated with pulmonary inflammatory diseases