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目的研究早期妊娠、晚期妊娠的胎盘组织、葡萄胎和绒癌组织中高迁移率族蛋白1(HMGB1)和核因子-κBp65(NF-κBp65)的表达变化及意义,探讨HMGB1和NF-κBp65与滋养细胞疾病的关系。方法选择2010年6月至2013年12月潍坊市中医院和潍坊市人民医院收治的流产、剖宫产、葡萄胎和绒癌标本,应用免疫组织化学方法检测HMGB1及NF-κBp65在早期妊娠、晚期妊娠的胎盘组织、葡萄胎和绒癌组织中的表达变化。结果 1.HMGB1阳性颗粒主要定位于细胞质和细胞核中,主要表达于绒毛滋养细胞和血管内皮细胞和间质细胞。与早期和晚期妊娠绒毛组织相比较,葡萄胎和绒癌组织中HMGB1阳性着色强度均明显增强,差异有统计学意义(P<0.001)。2.NF-κBp65阳性颗粒主要定位在细胞质中,主要分布于绒毛滋养细胞、绒毛间质、血管内皮细胞。与早期和晚期妊娠绒毛组织相比较,葡萄胎和绒癌组织中NF-κBp65阳性着色强度均明显增强,差异有统计学意义(P<0.001)。3.HMGB1与NF-κBp65平均光密度值在葡萄胎中表达强度呈正相关(r=0.7499,P=0.000);在绒癌组织中表达强度呈正相关(r=0.7338,P=0.000)。结论 HMGB1及NF-κBp65高表达与葡萄胎、绒癌的发生相关;HMGB1和NF-κBp65在葡萄胎和绒癌组织中的表达趋势一致,两者线性相关分析为正相关,提示HMGB1高表达可增强NF-κBp65的表达,与肿瘤细胞的生长有关。
Objective To investigate the expression and significance of HMGB1 and NF-κBp65 in placenta, hydatidiform mole and choriocarcinoma of early pregnancy and late pregnancy, and to explore the role of HMGB1 and NF-κBp65 in nourishing The relationship between cell diseases. Methods The samples of abortion, cesarean section, hydatidiform mole and choriocarcinoma admitted to Weifang Hospital of Traditional Chinese Medicine and Weifang People’s Hospital from June 2010 to December 2013 were detected by immunohistochemical method. The expressions of HMGB1 and NF-κBp65 in early pregnancy, Changes of placenta, hydatidiform mole and choriocarcinoma in late pregnancy. HGBGB1 positive particles mainly located in the cytoplasm and nucleus, mainly expressed in villous trophoblasts and vascular endothelial cells and interstitial cells. HMGB1 positive staining intensity in hydatidiform mole and choriocarcinoma tissues were significantly higher than those in early and late pregnancy, the difference was statistically significant (P <0.001). 2. NF-κBp65 positive particles mainly located in the cytoplasm, mainly distributed in villous trophoblast, villus stroma, vascular endothelial cells. The staining intensity of NF-κBp65 in hydatidiform mole and choriocarcinoma was significantly higher than that in early and late pregnancy (P <0.001). There was a positive correlation between HMGB1 and NF-κB p65 expression intensity in hydatidiform mole (r = 0.7499, P = 0.000). The expression intensity of HMGB1 was positively correlated with choriocarcinoma (r = 0.7338, P = 0.000). Conclusion The high expressions of HMGB1 and NF-κBp65 are correlated with the occurrence of hydatidiform mole and choriocarcinoma. The expressions of HMGB1 and NF-κBp65 are consistent in hydatidiform mole and choriocarcinoma tissues. The linear correlation analysis between HMGB1 and NF- Enhance the expression of NF-κBp65, and the growth of tumor cells.