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Objective To research on the optimal approach for the production of virus-free bulbs of lily.Methods Researches on the shoot tip size of lily,heat treatment and ribavirin concentration were carried out.The elimination efficiencies of five lily verities to the viruses of LVX,CMV and LSV were researched by four different culture methods,which were the shoot tip culture,the shoot tip culture combined with heat treatment,the shoot tip culture combined with ribavirin,and the shoot tip culture combined with heat treatment and ribavirin.Results Considering the survival rate and virus-free rate,single shoot tip culture could hardly eliminate the viruses.And the method of shoot tip culture (0.5-0.8 mm) combined with heat treatment (changing temperature treatment) and ribavirin (10 mg/L) showed the optimal elimination efficiencies.Conclusions The optimal approach for the production of virus-free bulbs of lily was researched,which provided references for the further development and utilization of lily.
Objective To research on the optimal approach for the production of virus-free bulbs of lily. Methods Researches on the shoot tip size of lily, heat treatment and ribavirin concentration were carried out. The elimination efficiencies of five lily verities to the viruses of LVX, CMV and LSV were researched by four different culture methods, which were the shoot tip culture, the shoot tip culture combined with heat treatment, the shoot tip culture combined with ribavirin, and the shoot tip culture combined with heat treatment and ribavirin. Results consider the the survival rate and virus-free rate, single shoot tip culture could hardly eliminate the viruses. And the method of shoot tip culture (0.5-0.8 mm) combined with heat treatment (changing temperature treatment) and ribavirin (10 mg / L) showed the optimal elimination efficiencies. Conclusions The optimal approach for the production of virus-free bulbs of lily was researched, which provided references for the further development and utilization of lily.