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目的探讨体内心肌微环境及体外5-氮杂胞苷(5-aza)诱导脂肪间充质干细胞分化为心肌样细胞的作用差异。方法取小鼠腹股沟的皮下脂肪组织,分离培养脂肪间充质干细胞(ADMSCs),对其进行表面标记和成骨、成脂分化能力鉴定。选取生长状态良好的第3代ADMSCs,分为5-aza体外诱导组、体内心肌移植组,体外诱导3周以及体内移植1周后,采用免疫荧光技术检测特异性心肌肌钙蛋白T(cT nT)的表达。结果两种诱导方法ADMSCs均表达cT nT,5-aza诱导组3周表达率(33.33±3.79)%,移植组1周表达率(42.93±4.04)%,移植组1周较5-aza诱导组3周具有更高的分化效率(P<0.05)。结论体外5-aza化学诱导和体内心肌微环境均可使ADMSCs分化为心肌样细胞,但心肌微环境的诱导分化效率明显高于5-aza的作用。
Objective To investigate the effects of in vitro myocardial microenvironment and in vitro differentiation of adipose-derived mesenchymal stem cells into cardiomyocyte-like cells induced by 5-azacytidine (5-aza). Methods The subcutaneous adipose tissue in the groin of mice was isolated and cultured. Adipose-derived mesenchymal stem cells (ADMSCs) were isolated and cultured, and their surface markers, osteogenic and adipogenic differentiation were identified. The third generation of ADMSCs with good growth status were selected and divided into 5-aza in vitro induction group and in vivo myocardial transplantation group. After induction for 3 weeks in vitro and in vivo for 1 week, the specific cardiac troponin T (cT nT )expression. Results The expression of cT nT was detected in both ADMSCs. The expression rate of 5-aza in induction group was 33.33 ± 3.79% (42.93 ± 4.04% 3 weeks had higher differentiation efficiency (P <0.05). Conclusions ADMSCs can differentiate into cardiomyocyte-like cells induced by 5-aza in vitro and in vivo myocardial microenvironment, but the efficiency of inducing and differentiating myocardial microenvironment is obviously higher than that of 5-aza.