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目的:探讨槲皮素和姜黄素对人肝癌HepG2细胞株增殖及凋亡的作用。方法:不同质量浓度的槲皮素(0、10、20、50、100μmol/L)和姜黄素(0、2、5、10、20μmol/L)分别作用HepG2细胞24、48、72 h,以As2O3(0、2、5、10、20μmol/L)为阳性对照,MTT法检测HepG2细胞的增殖,流式细胞术检测HepG2细胞的凋亡。结果:槲皮素、姜黄素及As2O3作用后,HepG2细胞形态发生变化、生长速率变慢,HepG2增殖率随着时间和药物浓度的增加逐渐减低,72 h时3种药物高浓度组HepG2细胞增殖率分别为(31±5.8)%、(51±4.6)%、(54±5.8)%。槲皮素和姜黄素均有明显的促凋亡作用,并呈时间和浓度依赖性;高浓度(100μmol/L)槲皮素作用最强,相同浓度下的姜黄素与As2O3作用相当。结论:槲皮素和姜黄素能明显抑制HepG2细胞增殖,促进其凋亡,并且这种作用强于As2O3。
Objective: To investigate the effects of quercetin and curcumin on proliferation and apoptosis of HepG2 human hepatoma cell line. METHODS: HepG2 cells were treated with different concentrations of quercetin (0, 10, 20, 50 and 100 μmol / L) and curcumin (0, 2, 5, 10 and 20 μmol / L) As2O3 (0, 2, 5, 10, 20μmol / L) was used as a positive control. The proliferation of HepG2 cells was detected by MTT assay. The apoptosis of HepG2 cells was detected by flow cytometry. Results: After treated with quercetin, curcumin and As2O3, the morphology of HepG2 cells changed and the growth rate became slower. The proliferation rate of HepG2 decreased gradually with the increase of time and drug concentration. After 72 h, the HepG2 cells were highly proliferated The rates were (31 ± 5.8)%, (51 ± 4.6)% and (54 ± 5.8)%, respectively. Quercetin and curcumin all showed obvious apoptosis-inducing effect in a time and concentration-dependent manner. Quercetin with the highest concentration (100μmol / L) had the strongest effect and curcumin at the same concentration had the same effect as As2O3. Conclusion: Quercetin and curcumin can significantly inhibit HepG2 cell proliferation and promote apoptosis, and this effect is stronger than that of As2O3.