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应用地高辛配基标记ＨＣＭＶＤＮＡＪ片断为探针，采用斑点杂交试验检测唾液及尿液中ＨＣＭＶＤＮＡ。此方法的最低检测阈值为０．５ｐｇ，与ＨＳＶ－１、ＨＳＶ－２、ＲｕＶ、ＤＮＡ无交叉反应。检测１７例患儿的唾液和尿液ＨＣＭＶＤＮＡ，其阳性率分别为５０％和１７．６％，唾液中ＨＣＭＶＤＮＡ阳性率显著高于尿液；检测１３６对正常母婴配对唾液标本，ＨＣＭＶＤＮＡ阳性率分别为４６．３％和９．６％，其相应血清用ＥＬＩＳＡ检测ＨＣＭＶ－ＩｇＭ抗体，阳性率分别为２．２％和０％。此结果提示，同时检测唾液中病毒ＤＮＡ及血清中ＩｇＭ抗体，对临床及流行病学筛检试验判断是否有激活感染及感染的早、晚期有重要意义。
Digoxigenin-labeled HCMVDNAJ fragment was used as a probe to detect HCMVDNA in saliva and urine by dot blot hybridization. The method has the lowest detection threshold of 0.5 pg and no cross-reaction with HSV-1, HSV-2, RuV, DNA. The positive rates of HCMVDNA in saliva and urine of 17 children were 50% and 17.6% respectively, and the positive rate of HCMVDNA in saliva was significantly higher than that in urine. The positive rate of HCMVDNA in 136 pairs of normal maternal and infant saliva samples was detected 46.3% and 9.6% respectively. The corresponding serum was detected by ELISA with HCMV-IgM antibody, the positive rates were 2.2% and 0% respectively. The results suggest that simultaneous detection of saliva DNA and serum IgM antibodies in clinical and epidemiological screening tests to determine whether the activation of infection and infection of early and late have important significance.