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为控制垂体移植免疫排斥．利用供体Ｗｉｓｔａｒ大鼠活化脾细胞（ＡＳＣ）和（或）环磷酰胺（ＣＰ）对去垂体ＳＤ大鼠进行免疫耐受诱导，同时检测受体Ｔ细胞业群、细胞因子及ＭＬＲ。结果ＡＳＣ５×１０７／０．５ｍｌ／周２次或ＡＳＣ联合ＣＰ２０ｍｇ／ｋｇ·ｄ２周，使移植皮片ＭＳＴ明显延长。致耐受体ＣＤ＋３、ＣＤ＋４、ＣＤ＋８细胞以及ＩＬ—２、ＩＬ——６和ＴＮＦ显著低于排斥组。ＭＬＲ显示，受体对特异抗原产生反应。表明ＡＳＣ和（或）ＣＰ能诱导去垂体大鼠的免疫耐受，其机制与Ｔ细胞业群对特定抗原的免疫无反应性及参予免疫应答的细胞因子水平下降有关。导师．Ｐ＜０．０１定［１２］ｒＩＬ一６标准品（法国Ｋｌｅｉｎ博士赠），四甲基偶素华盐（ＭＴＴ，瑞士）。１．７．３ＴＮＦ的检测：按ＴＮＦ敏感细胞株Ｌ９２９增殖法［１３］。ＴＮＦ标准品（法国Ｋｌｅｉｎ博士赠）。１．８混合淋巴细胞反应：取正常、去脑垂体及诱导耐受皮肤移植后的ＳＤ大鼠脾，制成脾细胞４×１０８／Ｌ作为反应细胞。取Ｗｉｓｔａｒ大鼠和ＢＡｂｌ／ｃ小鼠的经丝裂霉素处理的脾细胞８Ｘ１０／Ｌ作为刺激细胞。各组用含５％正常大鼠血清，１００ｍｇ／Ｌ庆大霉素，２５ｍｍｏｌＨＥＰＳ，２ｍｍｏｌ／ＬＬ一
To control pituitary graft rejection. Immunosuppression induced by donor splenocytes (ASC) and / or cyclophosphamide (CP) in donor Wistar rats was used to detect T cell subsets, cytokines and MLR in recipients. The results of ASC5 × 107 / 0.5ml / week twice or ASC combined with CP20mg / kg · d2 weeks, so that skin graft MST was significantly prolonged. Tolerant recipients CD + 3, CD + 4, CD + 8 cells and IL-2, IL-6 and TNF were significantly lower than the rejection group. MLR shows that the receptor responds to specific antigens. It is suggested that ASC and / or CP can induce the immune tolerance of the descending pituitary rats. The mechanism is related to the immune non-responsiveness of T-cell population to specific antigens and the decrease of cytokines involved in immune response. tutor. P <0.01 [12] rIL-6 standard (Dr. Klein, France), MTT (Switzerland). 1.7.3TNF detection: TNF-sensitive cell line L929 proliferation method [13]. TNF standard (France Dr. Klein gift). 1.8 mixed lymphocyte reaction: take normal, pituitary and SD induced spleen tolerance after transplantation, made of spleen cells 4 × 108 / L as a reactive cell. 8 × 10 / L mitomycin-treated splenocytes from Wistar rats and BAbl / c mice were used as stimulator cells. Each group containing 5% normal rat serum, 100mg / L gentamicin, 25mmolHEPS, 2mmol / LL a